The Publish button will create the eConnect node on your SQL Server and any associated SmartConnect entries when it is clicked. It will create a node in each company that was checked in the setup window and will add it to SmartConnect if those options were selected as well.
Note that if Node Builder has not been registered, then nodes will only be created in the Fabrikam test company when the node is published.
When a node is published, it creates the actual SQL Server stored procedure in each of the marked Dynamics GP companies. As the nodes are created, they will adjust the company databases used in Node Builder to match the company that the node is currently being created in.
This means there cannot be a node in Fabrikam that updates a table in the Subsidiary company. So if the node integrates into 2 tables in TWO (Fabrikam) and 1 table in TEST (some other database), when the node is created in Fabrikam it will still integrate into 2 tables in TWO and 1 in TEST. When the node is created in the Subsidiary company (SUB), however, it will integrate into 2 tables in SUB and 1 in Test.
If you choose to create a new Node Group or Type you will need to restart Dynamics GP on the client to see the new Node Groups or Types appear. Additionally, with the new eConnect Service on Dynamics GP 2010 you may need to restart the eConnect Service if you add additional parameters to your Node as the new service attempts to cache the parameters.